BackgroundTumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
DescriptionAPC Recombinant Monoclonal Antibody [5F2]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman, Rat
IsotypeRabbit IgG
Uniprot IDP25054
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human APC
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesAdenomatous polyposis coli protein (Protein APC) (Deleted in polyposis 2.5), APC, DP2.5
ApplicationELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200