BackgroundAbundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.
DescriptionGzma Polyclonal Antibody, Biotin Conjugated. Biotin. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityMouse
IsotypeIgG
Uniprot IDP11032
Purification>95%, Protein G purified
ImmunogenRecombinant Mouse Granzyme A protein (29-260AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesGranzyme A (EC:3.4.21.78), Gzma, Ctla-3, Ctla3, Mtsp-1, Autocrine thymic lymphoma granzyme-like serine protease, CTLA-3, Fragmentin-1, T cell-specific serine protease 1, TSP-1
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000