BackgroundAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
DescriptionHP Recombinant Monoclonal Antibody [5A5]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP00738
PurificationAffinity-chromatography
ImmunogenA synthesized peptide derived from human Haptoglobin
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesHaptoglobin (Zonulin) [Cleaved into: Haptoglobin alpha chain, Haptoglobin beta chain], HP
ApplicationELISA, IHC; Recommended dilution: IHC:1:50-1:200