BackgroundThis receptor tyrosine kinase responds to insulin by phosphorylating intracellular substrates like IRS proteins and SHC. These phosphorylated sites serve as docking points for other signaling proteins. Two main pathways are activated: the PI3K-AKT pathway, facilitating glucose transport and cell survival, and the Ras-MAPK pathway, influencing cell growth and differentiation. Activation of the mTORC1 pathway and interaction with IGF receptors are also regulated by this kinase. Different isoforms have distinct binding affinities for insulin and IGFs, influencing diverse cellular responses.
DescriptionINSR Recombinant Monoclonal Antibody [4B2]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP06213
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Insulin Receptor
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesInsulin receptor (IR) (EC 2.7.10.1) (CD antigen CD220) [Cleaved into: Insulin receptor subunit alpha, Insulin receptor subunit beta], INSR
ApplicationELISA, IF; Recommended dilution: IF:1:20-1:200