BackgroundRequired for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.
DescriptionlepA Polyclonal Antibody, HRP Conjugated. HRP. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityEscherichia coli
IsotypeIgG
Uniprot IDP60785
Purification>95%, Protein G purified
ImmunogenRecombinant Escherichia coli Elongation factor 4 protein (1-599AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesElongation factor 4, Ribosomal back-translocase LepA, lepA
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000