BackgroundActs as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
DescriptionNUF2 Recombinant Monoclonal Antibody [8B7]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDQ9BZD4
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human NUF2
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesKinetochore protein Nuf2 (hNuf2) (hNuf2R) (hsNuf2) (Cell division cycle-associated protein 1), NUF2, CDCA1 NUF2R
ApplicationELISA, WB; Recommended dilution: WB:1:500-1:2000