BackgroundKey kinase in intracellular signaling pathways from integrins and receptor-type kinases. Impacts cytoskeleton, cell adhesion, migration, proliferation, apoptosis, mitosis, and vesicle transport. Directly phosphorylates BAD for anti-apoptosis. Activated by CDC42 and RAC1, linking to JNK MAP kinase pathway. Phosphorylates MAP2K1, activating downstream MAP kinases. Regulates actin cytoskeleton, stress fibers, and focal adhesions. Controls tubulin chaperone TBCB, influencing microtubule organization. Affects insulin secretion and neuromuscular junction formation. Inhibited during apoptosis, binds CDC2L1/2. Phosphorylates MYL9/MLC2, RAF1, SNAI1, and more, with various impacts on cellular processes.
DescriptionPAK1 Recombinant Monoclonal Antibody [4F10]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman, Mouse
IsotypeRabbit IgG
Uniprot IDQ13153
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human PAK1
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesSerine/threonine-protein kinase PAK 1 (EC 2.7.11.1) (Alpha-PAK) (p21-activated kinase 1) (PAK-1) (p65-PAK), PAK1
ApplicationELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200