BackgroundThis gene encodes one of three closely related AKT serine-threonine protein kinases: protein kinase B alpha, beta, and gamma. These AKT proteins share a pleckstrin homology domain, a kinase domain, and a regulatory domain. They are phosphorylated by phosphoinositide 3-kinase (PI3K) and play pivotal roles in multiple signaling pathways involving membrane-bound ligands like receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. AKT proteins regulate diverse cellular functions such as proliferation, survival, metabolism, and angiogenesis in normal and malignant cells. Activated by phosphatidylinositol 3,4,5-trisphosphate (PIP3), AKT proteins require phosphorylation at threonine 308 and serine 473 for full activation. They are involved in the PI3K/AKT and mTOR signaling pathways, which control translation initiation and are implicated in cancer and other diseases. Mutations in this gene are linked to various cancers and conditions like Proteus syndrome and Cowden syndrome 6. Multiple transcript variants of this gene exist due to alternative splicing.
DescriptionPhospho-AKT1 (T450) Recombinant Monoclonal Antibody [2A4]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP31749
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Phospho-AKT1 (T450)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesRAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC
ApplicationELISA, WB; Recommended dilution: WB:1:500-1:5000