BackgroundSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
DescriptionPhospho-ERN1 (S724) Recombinant Monoclonal Antibody [1F12]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDO75460
PurificationAffinity-chromatography
ImmunogenA synthesized peptide derived from human Phospho-ERN1 (S724)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesSerine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, ERN1
ApplicationELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200