BackgroundThis serine/threonine protein kinase is involved in diverse signaling pathways, impacting cytoskeleton regulation, cell migration, growth, proliferation, and cell survival. Activation by growth factor receptors or active CDC42 and RAC1 triggers a conformational change and autophosphorylation on serine/threonine residues. It phosphorylates and inactivates SSH1, promoting cofilin's inhibitory phosphorylation, which stabilizes actin filaments. It also phosphorylates LIMK1, another cofilin inhibitor. Integrin beta5/ITGB5 phosphorylation controls cell motility, while ARHGEF2 phosphorylation activates RHOA, regulating focal adhesion and actin stress fiber assembly. For cell survival, it phosphorylates BAD, a BCL2 antagonist, or prevents caspase-8 binding to death domain receptors independently of its kinase activity to inhibit apoptosis. It affects cell-cycle progression by regulating CDKN1A levels and phosphorylating RAN.
DescriptionPhospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody [2D1]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDO96013/Q9P286/Q9NQU5
PurificationAffinity-chromatography
ImmunogenA synthesized peptide derived from human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesSerine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4, PAK4, KIAA1142
ApplicationELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200