BackgroundThis calcium- and diacylglycerol-independent serine/threonine-protein kinase operates in the PI3K pathway, MAP kinase cascade, NF-kappa-B activation, and mitogenic signaling. It influences cell polarity, proliferation, inflammatory response, and long-term potentiation (LTP). After stimuli like lipopolysaccharide or mitogens, it activates the MAPK1/ERK2 cascade, is vital for insulin-dependent AKT3 activation, and contributes to GLUT4 translocation in adipocytes. It plays roles in cell differentiation, inflammation, NF-kappa-B-mediated inflammatory response, and LTP maintenance. Also, it phosphorylates VAMP2 in vitro.
DescriptionPhospho-PRKCZ (T560) Recombinant Monoclonal Antibody [1B10]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDQ05513
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Phospho-PRKCZ (T560)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesProtein kinase C zeta type, nPKC-zeta, PRKCZ, PKC2
ApplicationELISA, WB; Recommended dilution: WB:1:500-1:5000