BackgroundThis gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
DescriptionPhospho-RAF1-S259 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityHuman
IsotypeIgG
Uniprot IDP04049
PurificationAffinity Purification
ImmunogenA synthetic phosphorylated peptide around S259 of human Phospho-Raf1-S259 (NP_002871.1).
StorageShipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles
Alternative NamesRAF1; CMD1NN; CRAF; NS5; Raf-1; c-Raf; Raf-1 proto-oncogene, serine/threonine kinase
ApplicationWB, IP; Recommended dilution: WB 1:500 - 1:2000, IP 1:50 - 1:100