BackgroundSerine/threonine-protein kinase linking Ras GTPases and MAPK/ERK cascade. Controls cell fate decisions like proliferation, differentiation, survival, and apoptosis. Initiates MAPK cascade. Phosphorylates key targets including BAD, adenylyl cyclases, PPP1R12A, and TNNT2. Regulates NF-kB, motility, apoptosis, proliferation, and angiogenesis. Involved in mitochondria-mediated cell protection, Rho signaling, wound healing, and oncogenic transformation.
DescriptionPhospho-RAF1 (S621) Recombinant Monoclonal Antibody [1C2]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP04049
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Phospho-RAF1 (S621)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesRAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF
ApplicationELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200