BackgroundCatalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
DescriptionRTCA Polyclonal Antibody, Biotin Conjugated. Biotin. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDO00442
Purification>95%, Protein G purified
ImmunogenRecombinant Human RNA 3'-terminal phosphate cyclase protein (167-379AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesRNA 3'-terminal phosphate cyclase (EC:6.5.1.4), RNA cyclase, RNA-3'-phosphate cyclase, RTCA, RPC, RPC1, RTC1, RTCD1, RNA terminal phosphate cyclase domain-containing protein 1, RTC domain-containing protein 1
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000