BackgroundThe RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.
DescriptionruvA Polyclonal Antibody, FITC Conjugated. FITC. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityEscherichia coli
IsotypeIgG
Uniprot IDP0A809
Purification>95%, Protein G purified
ImmunogenRecombinant Escherichia coli Holliday junction ATP-dependent DNA helicase RuvA protein (1-203AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesHolliday junction ATP-dependent DNA helicase RuvA, ruvA
ApplicationELISA; Recommended dilution: ELISA 1:100-1:500