BackgroundControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
DescriptionTOP2A Recombinant Monoclonal Antibody [5B10]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman, Mouse
IsotypeRabbit IgG
Uniprot IDP11388
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Topoisomerase II alpha
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesDNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme), TOP2A, TOP2
ApplicationELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200