BackgroundAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
DescriptionUBE2C Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4
SpecificityHuman
IsotypeIgG
Uniprot IDO00762
PurificationProtein G purified
ImmunogenRecombinant Human Ubiquitin-conjugating enzyme E2 C protein (2-179AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesUbiquitin-conjugating enzyme E2 C, (E3-independent) E2 ubiquitin-conjugating enzyme C , E2 ubiquitin-conjugating enzyme C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C, UBE2C, UBCH10
ApplicationELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200